The role of the endothelium-derived vasodilator, nitric oxide, as a mediator of the increase in gastric mucosal blood flow and as a modulator of the acid secretory response induced by pentagastrin was investigated in the anesthetised rat. Intravenous administration of the selective inhibitor of endogenous nitric oxide synthesis, NG-monomethyl-l-arginine (12.5 and 50 mg/kg), which dose-dependently increased systemic arterial blood pressure, did not affect resting acid output. However, NG-monomethyl-l-arginine significantly reduced resting gastric mucosal blood flow at the higher dose, as determined by hydrogen gas clearance. Infusion of pentagastrin (80 μg kg-1 · h-1) stimulated gastric acid secretion and elevated gastric mucosal blood flow. Pretreatment with NG-monomethyl-l-arginine (12.5 mg/kg IV) did not affect this stimulation of acid output but substantially attenuated (by 65% ± 10%; P < 0.01) the associated increase in gastric mucosal blood flow. Pretreatment with NG-monomethyl-l-arginine (50 mg/kg IV) induced a minor inhibition of pentagastrin-stimulated acid secretion but abolished the increase in gastric mucosal blood flow. When administered during pentagastrin infusion, NG-monomethyl-l-arginine (50 mg/ kg IV) did not affect the acid secretory response but induced a 76% ± 8% inhibition (P < 0.05) of the elevated gastric mucosal blood flow. The effects of NG-monomethyl-l-arginine on blood pressure, acid secretion, and gastric mucosal blood flow were abolished by pretreatment with the precursor for nitric oxide synthesis, l-arginine (300 mg/kg IV). These findings in the rat suggest that endogenous nitric oxide, synthesized from l-arginine, does not directly modulate the acid secretory response induced by pentagastrin but makes a substantial contribution to the mucosal vasodilatation associated with the stimulation of gastric acid secretion. © 1992.