DETECTION OF SHORT-CHAIN ALPHA-HYDROXYALDEHYDIC COMPOUNDS AS PENTAFLUORBENZYLOXIME DERIVATIVES IN BOVINE LIVER

被引：18

作者：

LOIDLSTAHLHOFEN, A ^{[1
]}

HANNEMANN, K ^{[1
]}

SPITELLER, G ^{[1
]}

机构：

[1] UNIV BAYREUTH,LEHRSTUHL ORGAN CHEM 1,D-95440 BAYREUTH,GERMANY

来源：

CHEMISTRY AND PHYSICS OF LIPIDS | 1995年 / 77卷 / 01期

关键词：

OXIDATIVE STRESS; SHORT-CHAIN ALPHA-HYDROXYALDEHYDES; QUANTIFICATION; (N-1)-HYDROXY-N-OXO ACIDS;

D O I：

10.1016/0009-3084(95)02459-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pentafluorbenzyloxime derivatization allows fast, gentle and unambiguous identification of alpha-hydroxyaldehydic lipid peroxidation products via GC/MS in biological material. Even 1.5 g of a bovine liver sample is sufficient to detect short-chain 2-hydroxyalkanales resulting from cleavage reactions of dioxygenated fatty acids. Quantification is achieved after secondary derivatization with N-methyl-N-t-butyldimethylsilyltrifluoracetamide (M-t-BSTFA) by mass spectrometry using characteristic ion traces of the derivatives. In addition, the corresponding (n-1)-hydroxy-n-oxo acids, previously unknown in biological material, could be detected.

引用

页码：113 / 119

页数：7

共 37 条

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