TERTIARY STRUCTURE DETERMINANTS IN TRANSFER RNA .I. PSEUDOURIDINE

被引：9

作者：

MILLAR, DB

机构：

[1] Laboratory of Physical Biochemistry, Naval Medical Research Institute, National Naval Medical Center, Bethesda, MD

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 174卷 / 01期

关键词：

D O I：

10.1016/0005-2787(69)90227-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The structural perturbation effects resulting from the cyanoethylation of Escherichia coli transfer RNA (tRNA) have been studied. The results suggest that: 1. 1. The C-C-A terminus area in cyanoethylated tRNA is in a more exposed state than in native tRNA. 2. 2. Cyanoethylated tRNA is more susceptible to nuclease degradation than native. 3. 3. There is a loss of A-U base pairs in cyanoethylated tRNA. 4. 4. Cyanoethylated tRNA is capable of forming a compact tertiary conformation in Mg2+ but it is a different one than that obtaining for native tRNA. 5. 5. Cyanoethylated tRNA does not show the same Mg2+ induced cooperative absorbance melting profile as native tRNA. This is probably due to the altered tertiary conformation. © 1969.

引用

页码：32 / &

共 27 条

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