The molecular weight of the alfalfa mosaic virus (AMV) protein subunit was determined from amino acid analyses and specific staining for certain amino acids on peptide maps from tryptic and chymotryptic digests of the protein. A subunit molecular weight of 32,600 was confirmed by estimations of C-terminal and N-terminal residues and of the tryptophan content. AMV nucleic acid consisted of four components with sedimentation values of 24.3, 20.0, 17.3, and 12.7 S. The molecular weights of these components were estimated. © 1969.