METHIONYL-TRANSFER RNA-SYNTHETASE OF ESCHERICHIA-COLI - ZINC METALLOPROTEIN

被引：57

作者：

POSORSKE, LH ^{[1
]}

COHN, M ^{[1
]}

YANAGISAWA, N ^{[1
]}

AULD, DS ^{[1
]}

机构：

[1] HARVARD UNIV, SCH MED, DEPT BIOL CHEM, BOSTON, MA 02115 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 576卷 / 01期

关键词：

(Escherichia coli); Metalloprotein; methionyl-tRNA synthetase; Zn[!sup]2+[!/sup;

D O I：

10.1016/0005-2795(79)90491-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The native dimeric form of methionyl-tRNA synthetase of Escherichia coli contains two zinc atoms per dimer, one per subunit. The bound zinc is retained upon trypsin modification which yields a monomer with one zinc atom. The enzymatic activity of both the dimeric forms is reversibly inhibited by 1,10-phenanthroline but not by its non-chelating analogues. In addition, the native enzyme binds two Mn2+ per dimer with a binding constant of approx. 70 μM but no binding is observed with the trypsin-modified monomer. © 1979.

引用

页码：128 / 133

页数：6

共 25 条

[1] RNA-DEPENDENT DNA-POLYMERASE (REVERSE-TRANSCRIPTASE) FROM AVIAN-MYELOBLASTOSIS VIRUS - ZINC METALLOENZYME
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[6] DEOXYNUCLEOTIDE-POLYMERIZING ENZYMES OF CALF THYMUS GLAND .4. INHIBITION OF TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE BY METAL LIGANDS
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[7] ROLE OF ZN(II) IN TRANSCRIPTION BY T7 RNA-POLYMERASE
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[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1974, 60 (02) : 641 - 648
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[9] EUGLENA-GRACILIS DNA-DEPENDENT RNA-POLYMERASE .2. ZINC METALLOENZYME
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[10] MECHANISM OF ACTION OF METHIONYL-TRANSFER-RNA SYNTHETASE FROM ESCHERICHIA-COLI - EQUILIBRIUM-DIALYSIS STUDIES ON BINDING OF METHIONINE, ATP AND ATP-MG2+ BY NATIVE AND TRYPSIN MODIFIED ENZYMES
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