OVINE LACTOFERRIN - ISOLATION FROM COLOSTRUM AND CHARACTERIZATION

被引：12

作者：

BUCHTA, R ^{[1
]}

机构：

[1] AUSTRALIAN NATL UNIV,JOHN CURTIN SCH MED RES,DEPT IMMUNOL,CANBERRA,ACT 2601,AUSTRALIA

来源：

JOURNAL OF DAIRY RESEARCH | 1991年 / 58卷 / 02期

关键词：

D O I：

10.1017/S0022029900029757

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Highly purified lactoferrin was isolated from ovine colostrum by sequential purification on CM-Sephadex C-50 and Blue-Sepharose, with overall yield of 55%. The ovine lactoferrin was characterized by SDS-PAGE, its amino acid composition and N-terminal sequence to residue 30. Homology with bovine and human lactoferrins was greater than 80 and 50% respectively. Antibodies to ovine lactoferrin were raised in rabbits and used to develop an enzyme-linked immunosorbent assay (ELISA). The antiserum was not cross reactive with other colostrum proteins.

引用

页码：211 / 218

页数：8

共 23 条

[1]

ABER A, 1979, EXPERIENTIA, V35, P1554

[2] LACTOFERRIN ENHANCES HYDROXYL RADICAL PRODUCTION BY HUMAN-NEUTROPHILS, NEUTROPHIL PARTICULATE FRACTIONS, AND AN ENZYMATIC GENERATING-SYSTEM [J].

AMBRUSO, DR ;

JOHNSTON, RB .

JOURNAL OF CLINICAL INVESTIGATION, 1981, 67 (02) :352-360

[3]

BAGGIOLINI M, 1970, Journal of Experimental Medicine, V131, P559, DOI 10.1084/jem.131.3.559

[4]

BANYARD MRC, 1982, MOL CELL BIOCHEM, V47, P115

[5] ISOLATION AND CHARACTERIZATION OF LACTOFERRIN SEPARATED FROM HUMAN WHEY BY ADSORPTION CHROMATOGRAPHY USING CIBACRON BLUE F3G-A LINKED AFFINITY ADSORBENT [J].

BEZWODA, WR ;

MANSOOR, N .

CLINICA CHIMICA ACTA, 1986, 157 (01) :89-93

[6] ISOLATION OF LACTOFERRIN FROM HUMAN WHEY BY A SINGLE CHROMATOGRAPHIC STEP [J].

BLACKBERG, L ;

HERNELL, O .

FEBS LETTERS, 1980, 109 (02) :180-184

[7] FUNCTIONAL AND BIOCHEMICAL-PROPERTIES OF OVINE NEUTROPHILS [J].

BUCHTA, R .

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 1990, 24 (02) :97-112

[8] IRON-BINDING PROTEINS IN MILK AND RESISTANCE TO ESCHERICHIA-COLI INFECTION IN INFANTS [J].

BULLEN, JJ ;

ROGERS, HJ ;

LEIGH, L .

BMJ-BRITISH MEDICAL JOURNAL, 1972, 1 (5792) :69-+

[9] ISOLATION OF LACTOFERRIN AND ITS CONCENTRATION IN SOWS COLOSTRUM AND MILK DURING A 21-DAY LACTATION [J].

ELLIOT, JI ;

SENFT, B ;

ERHARDT, G ;

FRASER, D .

JOURNAL OF ANIMAL SCIENCE, 1984, 59 (04) :1080-1084

[10]

GENNARO R, 1978, P SOC EXP BIOL MED, V157, P342, DOI 10.3181/00379727-157-40050

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