MOLECULAR-CLONING OF PHOSPHOFRUCTOKINASE-1 GENE FROM A THERMOPHILIC BACTERIUM, THERMUS-THERMOPHILUS

被引：20

作者：

XU, J ^{[1
]}

SEKI, M ^{[1
]}

DENDA, K ^{[1
]}

YOSHIDA, M ^{[1
]}

机构：

[1] TOKYO INST TECHNOL,DEPT LIFE SCI,YOKOHAMA,KANAGAWA 227,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 176卷 / 03期

关键词：

D O I：

10.1016/0006-291X(91)90429-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phosphofructokinase 1 (PFK1) from Thermus thermophilus differs from other bacterial PFKs in that it is regulated by effector-induced reversible tetramer-dimer conversion rather than conformational change alone. We have cloned its gene and the deduced amino acid sequence was compared with that of other PFKs. While almost all amino acid residues involved in substrate binding sites, which are assigned from crystal structures of other PFKs, are well conserved, some possibly important changes are found at subunit interfaces. © 1991.

引用

页码：1313 / 1318

页数：6

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