SPECIATION OF LACTIC-ACID BACTERIA FROM WINES BY HYBRIDIZATION WITH DNA PROBES

被引：25

作者：

LONVAUDFUNEL, A

FREMAUX, C

BITEAU, N

JOYEUX, A

机构：

[1] Institut d'OEnologie-Universite ́de Bordeaux II, 33405 Talence Cedex

来源：

FOOD MICROBIOLOGY | 1991年 / 8卷 / 03期

关键词：

D O I：

10.1016/0740-0020(91)90053-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The identification of lactic acid bacteria isolated from grape musts and wines was attempted using DNA probes. Total DNA extracted from the referenced strains was labelled with32P. Total DNA from the strains to be identified was spotted on filter membranes. Autoradiography, after prehybridization, hybridization and washing, revealed DNA samples homologous to the DNA probe. DNA identification proved to be faster and more reliable than biochemical tests for most of the species assayed. Even subspecies ofLactobacillus casei (subspeciesrhamnosus and subspeciescasei) could be distinguished. However, identification of oneLactobacillus brevis strain failed as its DNA hybridized with a strain ofLactobacillus hilgardii. For other strains, specific detection of species in the mixture of the two could be achieved. © 1991 Academic Press Limited.

引用

页码：215 / 222

页数：8

共 23 条

[1]

Carr, The Occurrence and Role of Lactic Acid Bacteria in Cider Making, PhD Thesis University of Bristol, (1956)

[2]

Delley, Mollet, Hottinger, DNA probe forLactobacillus delbrueckii, Appl. Environ. Microbiol., 56, pp. 1967-1970, (1990)

[3]

Dicks, Van Vuuren, Identification and physiological characteristics of heterofermentative strains of lactobacillus from South African red wines, Journal of Applied Bacteriology, 64, pp. 505-513, (1988)

[4]

Garvie, Hybridization between deoxyribonucleic acids of some strains of heterofermentative lactic acid bacteria, Int. J. Syst. Bac., 26, pp. 116-122, (1976)

[5]

Garvie, Farrow, The differentiation ofLeuconostoc oenos from non-acidophilic species of Leuconostoc, and the identification of five strains from the American Type Culture Collection, Am. J. Enol. Vitic., 31, pp. 154-157, (1980)

[6]

Gasson, Davies, High frequency conjugation associated withS. Lactis down cell aggregation, J. Bacteriol., 33, pp. 1260-1264, (1980)

[7]

Hontebeyrie, Gasser, Deoxyribonucleic acid homologies in the genus leuconostoc, Int. J. Syst. Bact., 27, pp. 9-14, (1977)

[8]

Irwin, Subden, Lautensach, Cunningham, Genetic heterogeneity in lactobacillii and leuconostocs of enological significance, Canadian Institute of Food Science and Technology Journal, 16, pp. 79-81, (1983)

[9]

Kandler, Weiss, Regular, Non-sporing Gram-positive rods, Bergey's Manual of Systematic Bacteriology, pp. 1208-1260, (1986)

[10]

Lafon-Lafourcade, Wine and brandy, Biotechnology, 5, pp. 81-163, (1983)

← 1 2 3 →