QUANTITATIVE-ANALYSIS OF ADSORBED SERUM-ALBUMIN ON SEGMENTED POLYURETHANE USING FT-IR/ATR SPECTROSCOPY

被引：38

作者：

JEON, JS

SPERLINE, RP

RAGHAVAN, S

机构：

[1] UNIV ARIZONA,DEPT MAT SCI & ENGN,TUCSON,AZ 85712

[2] UNIV ARIZONA,DEPT CHEM,TUCSON,AZ 85712

来源：

APPLIED SPECTROSCOPY | 1992年 / 46卷 / 11期

关键词：

INFRARED; ATTENUATED TOTAL REFLECTION; SURFACE; PROTEIN; ADSORPTION;

D O I：

10.1366/0003702924926826

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

FT-IR attenuated total reflection (ATR) spectroscopy was applied to quantitatively determine the extent of bovine serum albumin adsorbed onto a biomedical-grade poly(ether)urethane film deposited on a ZnSe internal reflection element (IRE). The method of adsorption density determination was based on the optical principles for a stratified medium consisting of three layers. The spectral peak area due to bulk solution was less than 1% of total peak area. The measured adsorption density of albumin in a flowing system was 3.9 mug/cm2 at a solution concentration of 4.5 g/100 mL.

引用

页码：1644 / 1648

页数：5

共 33 条

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[2]

BELLISSIMO JA, 1984, T AM SOC ART INT ORG, V30, P359

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