CELL-SURFACE-ASSOCIATED POLYPEPTIDES CSHA AND CSHB OF HIGH-MOLECULAR-MASS ARE COLONIZATION DETERMINANTS IN THE ORAL BACTERIUM STREPTOCOCCUS-GORDONII

The human oral bacterium Streptococcus gordonii expresses, on the cell surface, two-antigenically related high-molecular-mass polypeptides denoted CshA and CshB, encoded by genes at separate chromosomal loci. The precursor form of CshA is composed of four distinct segments: (i) a 41-amino-acid residue leader peptide, (ii) N-terminal 42-878 residues, (iii) residues 879-2417 comprising 13 repeat blocks of 101 amino acid residues and three shorter blocks, and (iv) a C-terminal anchor domain similar to those present in some other Gram-positive bacterial cell-wall polypeptides. Insertional mutations within cshA reduced both cell-surface hydrophobicity and ability to adhere to oral Actinomyces naeslundii. Insertional mutations in cshB had less effect on hydrophobicity and coadherence. However, expression of both polypeptides was found to be necessary for streptococci to colonize the murine oral cavity.