NOVEL ISOFORMS OF HUMAN CYCLIC AMP-RESPONSIVE ELEMENT MODULATOR (HCREM) MESSENGER-RNA

被引：14

作者：

FUJIMOTO, T ^{[1
]}

FUJISAWA, J ^{[1
]}

YOSHIDA, M ^{[1
]}

机构：

[1] UNIV TOKYO,INST MED SCI,DEPT CELLULAR & MOLEC BIOL,MINATO KU,TOKYO 108,JAPAN

来源：

JOURNAL OF BIOCHEMISTRY | 1994年 / 115卷 / 02期

关键词：

CRE-BINDING PROTEIN; CREM CDNA ISOFORM; HUMAN CREM;

D O I：

10.1093/oxfordjournals.jbchem.a124332

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cyclic AMP-response element (CRE), a transcriptional enhancer, is regulated by CREB (CRE-binding protein) which is the leucine zipper protein phosphorylated by protein kinase A in response to cAMP signal. The highly homologous protein CREM (CRE-modulator) is thought to modulate CREB-stimulated transcription, and is also involved in transcriptional control during spermatogenesis. In this paper, we report two types of cDNAs of human CREM (hCREM), type 1 and type 2; type 1 is a group of human counterparts of the mouse CREM alpha and type 2 is a novel form having a distinct 5' exon which is unrelated to any species of the CREB and CREM isoforms so far described. This unique 5' region of type 2 hCREM may suggest its independent expression from type 1 CREM. The specific 5' region of type 2 hCREM consisted of 88 bp, containing an initiation codon for translation, but no possible phosphorylation site, suggesting different roles from type 1 CREM. Both type 1 and 2 hCREMs are expressed in lymphoid and non-lymphoid cell lines. Their excess expression by transfection induced suppression of cAMP-mediated activation of transcription, suggesting their negative regulation of CRE-mediated transcription.

引用

页码：298 / 303

页数：6

共 24 条

[1]

BERGER SL, 1987, METHOD ENZYMOL, V152, P227

[2] ALTERNATIVE USAGE OF INITIATION CODONS IN MESSENGER-RNA ENCODING THE CAMP-RESPONSIVE-ELEMENT MODULATOR GENERATES REGULATORS WITH OPPOSITE FUNCTIONS [J].

DELMAS, V ;

LAOIDE, BM ;

MASQUILIER, D ;

DEGROOT, RP ;

FOULKES, NS ;

SASSONECORSI, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (10) :4226-4230

[3] DEVELOPMENTAL SWITCH OF CREM - FUNCTION DURING SPERMATOGENESIS - FROM ANTAGONIST TO ACTIVATOR [J].

FOULKES, NS ;

MELLSTROM, B ;

BENUSIGLIO, E ;

SASSONECORSI, P .

NATURE, 1992, 355 (6355) :80-84

[4] CREM GENE - USE OF ALTERNATIVE DNA-BINDING DOMAINS GENERATES MULTIPLE ANTAGONISTS OF CAMP-INDUCED TRANSCRIPTION [J].

FOULKES, NS ;

BORRELLI, E ;

SASSONECORSI, P .

CELL, 1991, 64 (04) :739-749

[5] TRANSCRIPTIONAL ANTAGONIST CAMP-RESPONSIVE ELEMENT MODULATOR (CREM) DOWN-REGULATES C-FOS CAMP-INDUCED EXPRESSION [J].

FOULKES, NS ;

LAOIDE, BM ;

SCHLOTTER, F ;

SASSONECORSI, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (12) :5448-5452

[6] A UNIQUE ENHANCER ELEMENT FOR THE TRANS ACTIVATOR (P40TAX) OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I THAT IS DISTINCT FROM CYCLIC AMP- AND 12-O-TETRADECANOYLPHORBOL-13-ACETATE-RESPONSIVE ELEMENTS [J].

FUJISAWA, J ;

TOITA, M ;

YOSHIDA, M .

JOURNAL OF VIROLOGY, 1989, 63 (08) :3234-3239

[7] A TRANSCRIPTIONAL ENHANCER SEQUENCE OF HTLV-I IS RESPONSIBLE FOR TRANSACTIVATION MEDIATED BY P40X OF HTLV-I [J].

FUJISAWA, J ;

SEIKI, M ;

SATO, M ;

YOSHIDA, M .

EMBO JOURNAL, 1986, 5 (04) :713-718

[8] A CLUSTER OF PHOSPHORYLATION SITES ON THE CYCLIC AMP-REGULATED NUCLEAR FACTOR CREB PREDICTED BY ITS SEQUENCE [J].

GONZALEZ, GA ;

YAMAMOTO, KK ;

FISCHER, WH ;

KARR, D ;

MENZEL, P ;

BIGGS, W ;

VALE, WW ;

MONTMINY, MR .

NATURE, 1989, 337 (6209) :749-752

[9]

GUBBLER U, 1983, GENE, V25, P263

[10] C-JUN DIMERIZES WITH ITSELF AND WITH C-FOS, FORMING COMPLEXES OF DIFFERENT DNA-BINDING AFFINITIES [J].

HALAZONETIS, TD ;

GEORGOPOULOS, K ;

GREENBERG, ME ;

LEDER, P .

CELL, 1988, 55 (05) :917-924

← 1 2 3 →