AMPLIFICATION OF MITOCHONDRIAL-DNA FROM ARCHIVAL TEMPORAL BONE SPECIMENS

被引：16

作者：

SIMPSON, TA ^{[1
]}

SMITH, RJH ^{[1
]}

机构：

[1] UNIV IOWA HOSP & CLIN,DEPT OTOLARYNGOL HEAD & NECK SURG,MOLEC OTOLARYNGOL RES LABS,IOWA CITY,IA 52242

来源：

LARYNGOSCOPE | 1995年 / 105卷 / 01期

关键词：

D O I：

10.1288/00005537-199501000-00009

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Archival temporal bone collections are an invaluable resource for studying the molecular genetics of many types of otopathology. The irreplaceable nature of temporal bone sections makes efficiency of DNA extraction of paramount importance. Several protocols are available for extracting DNA from fresh and preserved tissue. To establish the best protocol for reliably extracting DNA from celloidin-embedded temporal bone sections, a variety of DNA extraction techniques were tested. Using the optimum protocol, mitrochondrial DNA fragments ranging in size from less than 100 base pairs to more than 400 base pairs were amplified, and the authenticity of polymerase chain reaction (PCR) products was confirmed through comparative sequence analysis.

引用

页码：28 / 34

页数：7

共 18 条

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