THE PRESENCE OF A CALCIUM-SENSITIVE P26-CONTAINING COMPLEX IN BOVINE RETINA ROD CELLS

被引：37

作者：

GORODOVIKOVA, EN ^{[1
]}

PHILIPPOV, PP ^{[1
]}

机构：

[1] AN BELOZERSKY PHYSICOCHEM BIOL INST,DEPT ENZYMOL,MOSCOW 119899,RUSSIA

来源：

FEBS LETTERS | 1993年 / 335卷 / 02期

关键词：

PHOTORECEPTION; RECOVERIN; GUANYLATE CYCLASE; RHODOPSIN KINASE; BOVINE RETINA ROD CELL;

D O I：

10.1016/0014-5793(93)80746-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A protein with apparent M(r)26 kDa (p26[(1991) Biokimya 56, 225-228] or recoverin [(1991) Science 251, 915-918]) was suggested to activate GC in a calcium dependent manner in bovine retina rod cells [(1991) Science 251, 915-918;(1991) EMBO J, 10, 793-798] However, according to our present data homogeneous p26 preparations do not activate the enzyme. At the same time we have revealed a complex of p26 with an unidentified protein, presumably RK, in bovine ROS. Calcium favours formation of the complex whereas EGTA addition (which corresponds to a low free Ca2+ concentration) leads to its dissociation.

引用

页码：277 / 279

页数：3

共 18 条

[1]

ATON BR, 1984, BIOCHEMISTRY-US, V23, P1737, DOI 10.1021/bi00303a024

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3] MOLECULAR MECHANISM OF VISUAL TRANSDUCTION [J].

CHABRE, M ;

DETERRE, P .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 179 (02) :255-266

[4] RECOVERIN - A CALCIUM SENSITIVE ACTIVATOR OF RETINAL ROD GUANYLATE-CYCLASE [J].

DIZHOOR, AM ;

RAY, S ;

KUMAR, S ;

NIEMI, G ;

SPENCER, M ;

BROLLEY, D ;

WALSH, KA ;

PHILIPOV, PP ;

HURLEY, JB ;

STRYER, L .

SCIENCE, 1991, 251 (4996) :915-918

[5]

DIZHUR A M, 1991, Biokhimiya, V56, P225

[6]

FLEISCHMAN D, 1981, CURR TOP MEMBR TRANS, V15, P109

[7] GUANYLATE-CYCLASE FROM BOVINE ROD OUTER SEGMENTS - SOLUBILIZATION, PARTIAL-PURIFICATION, AND REGULATION BY INORGANIC PYROPHOSPHATE [J].

HAKKI, S ;

SITARAMAYYA, A .

BIOCHEMISTRY, 1990, 29 (04) :1088-1094

[8] PURIFICATION OF GUANYLYL CYCLASE FROM ROD OUTER SEGMENTS [J].

HORIO, Y ;

MURAD, F .

BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1133 (01) :81-88

[9] RECOVERINS ROLE - CONCLUSION WITHDRAWN [J].

HURLEY, JB ;

DIZHOOR, AM ;

RAY, S ;

STRYER, L .

SCIENCE, 1993, 260 (5109) :740-740

[10] RHODOPSIN PHOSPHORYLATION AS A MECHANISM OF CYCLIC-GMP PHOSPHODIESTERASE REGULATION BY S-MODULIN [J].

KAWAMURA, S .

NATURE, 1993, 362 (6423) :855-857

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