USE OF LIQUID SCINTILLATION QUENCHING TO QUANTITATE LIPID MASS

A new method for detecting 10-100 μg lipid mass in unmodified liquid scintillation spectrometers was developed by taking advantage of color quenching in such systems. The color, which quenches an isolated scintillation source, is produced by an H2SO4-lipid reaction carried out in a scintillation vial at 200°C for 60 min. The isolated source, a glass tube containing a mixture of plastic scintillator and β emitter (14C), is then inserted into the vial containing the colored solution for radioassay. The counting rate of the source is decreased as a linear function by the color intensity of the H2SO4-lipid solution. © 1969.