EVIDENCE FOR COMMUNICATION BETWEEN NERVE GROWTH-FACTOR AND PROTEIN TYROSINE PHOSPHORYLATION

被引：113

作者：

GOMEZ, N

TONKS, NK

MORRISON, C

HARMAR, T

COHEN, P

机构：

[1] UNIV WASHINGTON,DEPT BIOCHEM,SEATTLE,WA 98195

[2] UNIV EDINBURGH,MRC,BRAIN METAB UNIT,EDINBURGH EH8 9YL,MIDLOTHIAN,SCOTLAND

来源：

FEBS LETTERS | 1990年 / 271卷 / 1-2期

基金：

英国医学研究理事会;

关键词：

Nerve growth factor; Okadaic acid; PC12; cell; Protein kinase; Protein phosphatase; Tyrosine phosphorylation;

D O I：

10.1016/0014-5793(90)80386-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nerve growth factor (NGF) stimulation of PC12 cells activated two myelin basic protein (MBP) kinase activities > 10-fold within 5 min, which were resolved by chromatography on Mono Q. Each enzyme phosphorylated MBP on threonine and was inactivated by incubation with either CD45, a protein tyrosine phosphatase, or protein phosphatase 2A (PP2A), a serine/threonine phosphatase. The effects of CD45 and PP2A were prevented by vanadate and okadaic acid, respectively. Activation of the MBP-kinases provides a mechanism for communication between NGF and intracellular protein tyrosine phosphorylation. © 1990.

引用

页码：119 / 122

页数：4

共 19 条

[1] REQUIREMENT FOR INTEGRATION OF SIGNALS FROM 2 DISTINCT PHOSPHORYLATION PATHWAYS FOR ACTIVATION OF MAP KINASE [J].