IDENTIFICATION OF SUBREGIONS OF BORDETELLA-PERTUSSIS FILAMENTOUS HEMAGGLUTININ THAT STIMULATE HUMAN T-CELL RESPONSES

被引：16

作者：

DITOMMASO, A ^{[1
]}

DOMENIGHINI, M ^{[1
]}

BUGNOLI, M ^{[1
]}

TAGLIABUE, A ^{[1
]}

RAPPUOLI, R ^{[1
]}

DEMAGISTRIS, MT ^{[1
]}

机构：

[1] SCLAVO RES CTR,VIA FIORENTINA 1,I-53100 SIENA,ITALY

来源：

INFECTION AND IMMUNITY | 1991年 / 59卷 / 09期

关键词：

D O I：

10.1128/IAI.59.9.3313-3315.1991

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Filamentous hemagglutinin (FHA), a 220-kDa protein that mediates the adhesion of Bordetella pertussis to eukaryotic cells, is a component of acellular vaccines against whooping cough. To identify the subregions of FHA that are immunogenic for T cells, 16 human T-cell clones were raised against purified FHA and tested for the recognition of recombinant and proteolytic fragments. The clones were found to map either in the carboxy-terminal or the amino-terminal part of the FHA molecule, but none of them recognized the central region, which contains a sequence that is homologous to that of the eukaryotic protein fibronectin. These data suggest that subregions of FHA that do not contain sequences that are potentially cross-reactive with self proteins may be sufficient to induce an immune response against the whole protein.

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页码：3313 / 3315

页数：3

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