RETROGRADE LABELING OF DORSAL-ROOT GANGLION-CELLS IN THE RAT - A QUANTITATIVE AND MORPHOLOGICAL COMPARISON OF FLUOROGOLD WITH HORSERADISH-PEROXIDASE LABELING

被引：13

作者：

BARANOWSKI, AP ^{[1
]}

ANAND, U ^{[1
]}

MCMAHON, SB ^{[1
]}

机构：

[1] UNITED MED & DENT SCH GUYS & ST THOMAS HOSP,ST THOMAS HOSP,SCH MED,DEPT PHYSIOL,LONDON SE1 7EH,ENGLAND

来源：

NEUROSCIENCE LETTERS | 1992年 / 141卷 / 01期

基金：

英国惠康基金;

关键词：

SENSORY NEURON; DORSAL ROOT GANGLION CELL; NEUROANATOMICAL TRACING;

D O I：

10.1016/0304-3940(92)90332-2

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We have compared retrograde labelling of rat primary sensory neurons using Fluoro-Gold (FG) and horseradish peroxide conjugated with wheat germ agglutinin (HRP-WGA). Fluoro-Gold 2.5% after 48 h transit time and FG 5% after 24 and 48 h retrogradely labelled similar numbers of cell profiles as HRP-WGA (P > 0.1% Student's t-test). The calculated cell size distribution for the above FG groups were similar to those for the HRP-WGA. However, FG 2.5% after a 24 h transit time labelled significantly fewer cells (P < 0.001 Student's t-test). FG retrograde transport may be used to identify the same population of DRG cells as HRP-WGA.

引用

页码：53 / 56

页数：4

共 11 条

[1] SYMPATHETIC AND AFFERENT SOMATA PROJECTING IN HINDLIMB NERVES AND THE ANATOMICAL ORGANIZATION OF THE LUMBAR SYMPATHETIC NERVOUS-SYSTEM OF THE RAT [J].