ION TRAP DETECTOR - CAPILLARY GAS-CHROMATOGRAPHY OF VALPROIC ACID AND ITS MONOUNSATURATED METABOLITES IN SERUM USING METHYL-ESTER DERIVATIVES

被引：18

作者：

GAETANI, E ^{[1
]}

LAURERI, CF ^{[1
]}

VITTO, M ^{[1
]}

机构：

[1] UNIV PARMA,IST CHIM FARMACEUT & TOSSICOL,VIA M AZEGLIO 85,I-43100 PARMA,ITALY

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1992年 / 10卷 / 2-3期

关键词：

VALPROIC ACID; GC-MS; METHYL ESTER DERIVATIVES; MONOUNSATURATED METABOLITES;

D O I：

10.1016/0731-7085(92)80028-L

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A quantitative method was developed for valproic acid and five of its mono-unsaturated metabolites using capillary gas chromatography-mass spectrometry with selected ion monitoring. The method was applied to serum and all metabolites were measured in a single run. Methyl esters were synthesized as the derivatives suitable for gas chromatography. Calibration curves were found to be linear and the sensitivities in the order of 0.1-mu-g ml-1. Patients' data are presented. By this method it is possible to separate the stereoisomers of 2-n-propyl-2-pentenoic acid and of 2-n-propyl-3-pentenoic acid.

引用

页码：193 / 197

页数：5

共 20 条

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