METALLOCENE-MEDIATED AMPEROMETRIC BIOSENSORS FOR ONE-SHOT GLUCOSE DETERMINATION

Amperometric biosensors for the determination of glucose based on carbon black screen-printed on ceramics are described. The mediators, nickelocene, ferrocene and their derivatives, are used for the conjunction of glucose oxidase-catalysed oxidation of glucose and electrochemical reaction at the applied potential. It is shown that the nickelocene-mediated glucose oxidase electrode exhibits a similar behaviour to that mediated with ferrocene. However, the quasi-redox potential of nickelocene/nickelocenium cation, which is by 0.3 V more negative than that of any of the investigated ferrocene derivatives, significantly lowers the operating potential of the biosensor to almost-equal-to 0.00 V versus an Ag/AgCl electrode.