STIMULATION OF TRANSCYTOSIS OF THE POLYMERIC IMMUNOGLOBULIN RECEPTOR BY DIMERIC IGA

被引:86
作者
SONG, WX
BOMSEL, M
CASANOVA, J
VAERMAN, JP
MOSTOV, K
机构
[1] UNIV CALIF SAN FRANCISCO,DEPT BIOCHEM,SAN FRANCISCO,CA 94143
[2] UNIV CALIF SAN FRANCISCO,CARDIOVASC RES INST,SAN FRANCISCO,CA 94143
[3] UNIV CATHOLIQUE LOUVAIN,INT INST CELLULAR & MOLEC PATHOL,EXPTL MED UNIT,B-1200 BRUSSELS,BELGIUM
关键词
PHOSPHORYLATION; BIOTINYLATION; ENDOSOMES;
D O I
10.1073/pnas.91.1.163
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The polymeric immunoglobulin receptor (pIgR) is transcytosed from the basolateral to the apical surface of polarized epithelial cells. We have previously shown that phosphorylation of Ser-664 in the cytoplasmic domain of the pIgR is a signal for its transcytosis. We now report that binding of a physiological ligand, dimeric IgA, to pIgR stimulates pIgR transcytosis. This stimulation occurs in both the presence or absence of Ser-664 phosphorylation. We have used three methods to measure transcytosis of the pIgR. (i) The pIgR was biosynthetically labeled and its cleavage to secretory component after transcytosis was measured. (ii) The pIgR was labeled with biotin at the basolateral surface. After transcytosis, release of the biotin-labeled secretory component into the apical medium was measured. (iii) Transcytosis of a ligand bound to the pIgR was measured. All three methods indicated that dimeric IgA stimulates transcytosis of the pIgR.
引用
收藏
页码:163 / 166
页数:4
相关论文
共 22 条
[1]   THE POLYMERIC IMMUNOGLOBULIN RECEPTOR - A MODEL PROTEIN TO STUDY TRANSCYTOSIS [J].
APODACA, G ;
BOMSEL, M ;
ARDEN, J ;
BREITFELD, PP ;
TANG, K ;
MOSTOV, KE .
JOURNAL OF CLINICAL INVESTIGATION, 1991, 87 (06) :1877-1882
[2]  
APODACA G, 1993, J BIOL CHEM, V268, P23712
[3]  
AROETI B, 1992, INT REV CYTOL, V137B, P157
[4]  
BOMSEL M, 1991, Current Opinion in Cell Biology, V3, P647, DOI 10.1016/0955-0674(91)90036-X
[5]  
BRANDTZAEG P, 1981, CLIN EXP IMMUNOL, V44, P221
[6]  
BREITFELD PP, 1990, J BIOL CHEM, V265, P13750
[7]   POSTENDOCYTOTIC SORTING OF THE LIGAND FOR THE POLYMERIC IMMUNOGLOBULIN RECEPTOR IN MADIN-DARBY CANINE KIDNEY-CELLS [J].
BREITFELD, PP ;
HARRIS, JM ;
MOSTOV, KE .
JOURNAL OF CELL BIOLOGY, 1989, 109 (02) :475-486
[8]  
BREITFELD PP, 1989, METHOD CELL BIOL, V32, P329
[9]   PHOSPHORYLATION OF THE POLYMERIC IMMUNOGLOBULIN RECEPTOR REQUIRED FOR ITS EFFICIENT TRANSCYTOSIS [J].
CASANOVA, JE ;
BREITFELD, PP ;
ROSS, SA ;
MOSTOV, KE .
SCIENCE, 1990, 248 (4956) :742-745
[10]   MASSIVE PLASMA-CELL INFILTRATION OF THE DIGESTIVE-TRACT - SECRETORY COMPONENT AS THE RATE-LIMITING FACTOR OF IMMUNOGLOBULIN SECRETION IN EXTERNAL FLUIDS [J].
COLOMBEL, JF ;
RAMBAUD, JC ;
VAERMAN, JP ;
GALIAN, A ;
DELACROIX, DL ;
NEMETH, J ;
DUPREY, F ;
HALPHEN, M ;
GODEAU, P ;
DIVE, C .
GASTROENTEROLOGY, 1988, 95 (04) :1106-1113