RESISTANCE TO SKIN GRAFTS INDUCED BY CELL EXTRACTS IN CONGENIC STRAINS OF MICE DIFFERING FROM DONOR AT VARIOUS NON-H-2 LOCI

Cell extracts were prepared from C57BL/10 liver and spleen and tested for their capacity to immunize congenic lines, differing from C57BL/10 only at non-H-2 loci, against subsequent C57BL/10 skin grafts. Substantial and generally consistent immunization was obtained with spleen extraet in 9 of 14 lines, weak and irregular immunization in 4 (generally those with the weaker differences from C57BL/10), and none in 1. Immunization resulted from all three routes tested, i.v., i.p., and s.c., but was consistently stronger in mice injected by the last named route than in mice injected by the first two. Triple injections by either i.p. or s.c. routes were not consistently better than single injections, nor was there any obvious effect of total dose within the range 0.2-3 mg/mouse by the s.c. route and 1-4.5 mg by the i.p. route. By the i.p. route, 0.2 mg was possibly less effective than 1 mg. Although the immunity induced by the s.c. route was stronger than that induced by the other routes, it appeared more slowly. In the strain combination C57BL/10-B10. LP, it peaked at 7 days, disappeared at 10-11 days, reappeared at 14 days, and persisted to the end of the experiment at 56 days. Immunity seemed also to be cyclical in the weaker donor-recipient combination C57BL/10 28NX, with peaks and valleys appearing later. Immunity induced by the i.p. route persisted to at least day 28. In accordance with reports in the literature, liver extract was far less effective in inducing immunity than spleen extract and, in some cases, may have given rise to tolerance. Irradiation of spleen extract with 500 R of X-rays did not lower its activity; irradiation with 10,000 R substantially reduced but did not entirely destroy it. Cholate soluble and insoluble fractions prepared from the particulate fraction" both retained substantial activity for at least some of the non-H-2 antigens under test. © 1969 by The Williams and Wilkins Co."