SIMPLE BECKMAN DU ACCESSORY FOR LOCATION OF PROTEINS SEPARATED BY ISOELECTRIC-FOCUSING IN GRANULATED GEL LAYERS - ISOLATION OF HUMAN-SERUM VERY LOW-DENSITY APOLIPOPROTEIN-C-II AND APOLIPOPROTEIN-C-III-1

被引：16

作者：

HOLMQUIST, L

BROSTROM, H

机构：

[1] King Gustaf V Research Institute, Karolinska Hospital

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1979年 / 1卷 / 02期

关键词：

isoelectric focusing; isolation; location; proteins; very low density apolipoproteins;

D O I：

10.1016/0165-022X(79)90018-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple, low-cost gel layer scanner has been developed for the Beckman DU spectrophotometer. The gel scanner makes it possible to localize zones precisely after preparative isoelectric focusing of proteins in 2-mm thick Sephadex G-200 layers. Using the scanner after isoelectric separation of a mixture of human serum apolipoproteins C-III-1 and C-II, pure proteins could easily be obtained. © 1979.

引用

页码：117 / 127

页数：11

共 9 条

[1]

CARLSON K, 1973, J CLIN PATHOL S5, V26, P32

[2] SELECTIVE EXTRACTION OF HUMAN-SERUM VERY LOW-DENSITY APOLIPOPROTEINS WITH ORGANIC-SOLVENTS [J].