MOLECULAR HETEROGENEITY OF PREGNANCY SPECIFIC BETA-1 GLYCOPROTEIN - EFFECT ON MEASUREMENT BY RADIOIMMUNOASSAY AND ELECTRO-IMMUNOASSAY

被引：25

作者：

TEISNER, B

GRUDZINSKAS, JG

HINDERSSON, P

ALANI, ATM

WESTERGAARD, JG

CHARD, T

机构：

[1] ODENSE UNIV,DEPT OBSTET & GYNAECOL,DK-5000 ODENSE,DENMARK

[2] ST BARTHOLOMEWS HOSP,COLL MED,DEPT REPROD PHYSIOL,LONDON EC1A 7BE,ENGLAND

[3] ST BARTHOLOMEWS HOSP,COLL MED,DEPT OBSTET & GYNAECOL,LONDON EC1A 7BE,ENGLAND

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1979年 / 31卷 / 1-2期

关键词：

D O I：

10.1016/0022-1759(79)90294-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A comparison of methods for the quantification of circulating pregnancy specific β1 glycoprotein in late pregnancy was performed to assess the influence of the presence of a high molecular weight glycoprotein with α2 electrophoretic mobility (SP1α) which is immunochemically identifiable with pregnancy-specific β1 glycoprotein (SP1β). Serum samples from 47 volunteers in the 3rd trimester of pregnancy were subjected to measurements of pregnancy specific β1 glycoprotein in rocket immunoelectrophoresis, quantitative crossed-immunoelectrophoresis and radioimmunoassay. Rocket immunoelectrophoresis gives a result which reflects the total SP1 content, i.e. SP1α and SP1β while quantitative crossed-immunoelectrophoresis permits differentiation between the two molecules. Radioimmunoassay predominantly measures authenic SP1, i.e. SP1β in the presence of physiological amounts of SP1α. © 1979.

引用

页码：141 / 149

页数：9

共 11 条

[1] DETECTION AND CHARACTERIZATION OF PREGNANCY PROTEINS IN HUMAN PLACENTA AND THEIR QUANTITATIVE IMMUNOCHEMICAL DETERMINATION IN SERA FROM PREGNANT WOMEN [J].