PURIFIED OMEGA-CONOTOXIN GVIA RECEPTOR OF RAT-BRAIN RESEMBLES A DIHYDROPYRIDINE-SENSITIVE L-TYPE CALCIUM-CHANNEL

被引：113

作者：

MCENERY, MW

SNOWMAN, AM

SHARP, AH

ADAMS, ME

SNYDER, SH ^{[1
]}

机构：

[1] JOHNS HOPKINS MED INST, DEPT NEUROSCI, 725 N WOLFE ST, BALTIMORE, MD 21205 USA

[2] JOHNS HOPKINS MED INST, DEPT PHARMACOL & MOLEC SCI, BALTIMORE, MD 21205 USA

[3] JOHNS HOPKINS MED INST, DEPT PSYCHIAT & BEHAV SCI, BALTIMORE, MD 21205 USA

[4] UNIV CALIF RIVERSIDE, DEPT ENTOMOL, RIVERSIDE, CA 92521 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 24期

关键词：

VOLTAGE-DEPENDENT CALCIUM CHANNELS; OMEGA-AGATOXIN IIIA; PHOTOAFFINITY LABELING;

D O I：

10.1073/pnas.88.24.11095

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The omega-conotoxin GVIA (CTX) receptor has been purified 1900-fold to apparent homogeneity by monitoring both reversible binding of I-125-labeled CTX (I-125-CTX) and photoincorporation of N-hydroxysuccinimidyl-4-azidobenzoate-I-125-CTX (HSA-I-125-CTX). Photoincorporation of HSA-I-125-CTX into a 230-kDa protein exhibits a pharmacologic and chromatographic profile indicating that the 230-kDa protein is the CTX-binding subunit of the receptor. The pharmacologic specificity of I-125-CTX binding to the purified CTX receptor closely resembles that of the native membrane-bound form with respect to sensitivity towards CTX (K(d) = 32 pM) and other peptide toxin antagonists. The purified CTX receptor comprises the 230-kDa protein (alpha-1) and four additional proteins with apparent molecular masses of 140 (alpha-2), 110, 70 (beta-2), and 60 (beta-1) kDa. This subunit structure closely resembles that of the 1,4-dihydropyridine-sensitive L-type calcium channel.

引用

页码：11095 / 11099

页数：5

共 52 条

[1]

ABE T, 1987, J BIOL CHEM, V262, P9877

[2] SUBUNIT STRUCTURE AND LOCALIZATION OF DIHYDROPYRIDINE-SENSITIVE CALCIUM CHANNELS IN MAMMALIAN BRAIN, SPINAL-CORD, AND RETINA [J].