REACTIVATION OF DENATURED FUNGAL GLUCOSE-6-PHOSPHATE-DEHYDROGENASE AND ESCHERICHIA-COLI ALKALINE-PHOSPHATASE WITH ESCHERICHIA-COLI RIBOSOME

被引：39

作者：

DAS, B ^{[1
]}

CHATTOPADHYAY, S ^{[1
]}

DASGUPTA, C ^{[1
]}

机构：

[1] UNIV CALCUTTA,DEPT BIOPHYS MOLEC BIOL & GENET,92 APC RD,CALCUTTA 700009,W BENGAL,INDIA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 183卷 / 02期

关键词：

D O I：

10.1016/0006-291X(92)90550-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fungal glucose 6-phosphate dehydrogenase and E. coli alkaline phosphatase were denatured either by physical or by chemical means. In vitro reconstitution of these denatured enzymes was assisted by 70S E. coli ribosome, as shown by the recovery of their catalytic competence. Almost total recovery of the activities of completely inactivated enzymes was obtained when 70S ribosome was present at about equimolar concentration with the enzyme molecules at 37C and 50C, respectively. © 1992.

引用

页码：774 / 780

页数：7

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