THE SMT-0 MUTATION WHICH ABOLISHES MATING-TYPE SWITCHING IN FISSION YEAST IS A DELETION

被引：61

作者：

STYRKARSDOTTIR, U ^{[1
]}

EGEL, R ^{[1
]}

NIELSEN, O ^{[1
]}

机构：

[1] UNIV COPENHAGEN,INST GENET,OSTER FARIMAGSGADE 2A,DK-1353 COPENHAGEN,DENMARK

来源：

CURRENT GENETICS | 1993年 / 23卷 / 02期

关键词：

FISSION YEAST; MATING-TYPE SWITCHING; DOUBLE-STRAND BREAK; RECOMBINATION;

D O I：

10.1007/BF00352020

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Mating-type switching in the fission yeast, S. pombe, is initiated by a DNA double-strand break (DSB) between the mat1 cassette and the H1 homology box. The mat1-cis-acting mutant, smt-0, abolishes mating-type switching and is shown here to be a 263-bp deletion. This deletion starts in the middle of the H1 homology box, 31 bp from the site of the DSB, and extends into the flanking region distal to mat1. The sequence of the region distal to H1 in the wild-type is also presented. In this region we observe a bias in the distribution of purine residues between the two DNA strands.

引用

页码：184 / 186

页数：3

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