CDNA ANALYSIS OF THE MITE ALLERGEN LEP-D-1 IDENTIFIES 2 DIFFERENT ISOALLERGENS AND VARIANTS

被引：38

作者：

SCHMIDT, M ^{[1
]}

OLSSON, S ^{[1
]}

VANDERPLOEG, I ^{[1
]}

VANHAGEHAMSTEN, M ^{[1
]}

机构：

[1] E CAROLINA UNIV, SCH MED, DEPT PATHOL & LAB MED, GREENVILLE, NC 27834 USA

来源：

FEBS LETTERS | 1995年 / 370卷 / 1-2期

关键词：

ALLERGY; MITE; CDNA CLONING; PCR; POLYMORPHISM;

D O I：

10.1016/0014-5793(95)98164-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

For the first time the complete cDNA encoding two isoallergens of the non-pyroglyphid dust mite Lepidoglyphus destructor, Lep d 1, allergen has been sequenced. In addition, one of the isoallergens was found to have two variants, Oligonucleotides were designed from known amino acid sequences. The cDNA sequences were obtained by hybridizing these primers to mRNA and enhancement by the RT-PCR technique. To obtain the different complete encoding cDNA sequences and eliminate heteroduplex artifacts, we performed PCR + 1 reactions. Comparison of the amino acid sequence of the allergen shows leader sequences of 16 amino acids for both isoforms.

引用

页码：11 / 14

页数：4

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