OPTIMIZING METHYLATION CONDITIONS FOR GAS-LIQUID CHROMATOGRAPHY ASSAY OF LACTIC AND SUCCINIC ACID IN BIOLOGICAL SAMPLES

被引：17

作者：

BRICKNELL, KS ^{[1
]}

BROOK, I ^{[1
]}

FINEGOLD, SM ^{[1
]}

机构：

[1] VET ADM WADSWORTH HOSP CTR,MED SERV,LOS ANGELES,CA 90073

来源：

CHROMATOGRAPHIA | 1979年 / 12卷 / 01期

关键词：

D O I：

10.1007/BF02271594

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Aqueous standard solutions of lactic and succinic acids were methylated for assay by gas-liquid chromatography. The efficiency of methylation using boron trifluoridemethanol reagent was compared to methylation using a sulfuric acid-methanol mixture; the ratio of sulfuric acid and methanol was also varied. Reactions were done at 100°C/5 min and at room temperature/20 hours. We found the sulfuric acid-methanol was most efficient for a rapid assay; however, the ratio of sulfuric acid and methanol used is critical in the assay of lactic acid and would be especially important in the clinical laboratory using gas-liquid chromatography for analysis of cerebrospinal fluid from suspect meningitis patients or other body fluids. © 1979 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.

引用

页码：22 / 25

页数：4

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