HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF GLYCOALKALOIDS IN POTATO PRODUCTS

A method for the determination of α-solanine and α-chaconine, two major potato glycoalkaloids, in commercial potato products by high-performance liquid chromatography (HPLC) was developed. The glycoalkaloids were extracted with methanol and then purified using Sep-Pak C18 or Sep-Pak NH2 cartridges. A Nucleosil 5-NH2 column was employed for HPLC with acetonitrile-20 mM potassium dihydrogenphosphate (75:25, v/v) as the mobile phase. The calibration graph was linear in the range 1-50, μ g/ml for both α-solanine and α-chaconine. The average recoveries were 82.4-92.6% for α-solanine and 86.5-97.4% for α-chaconine added to various commercial potato products at a level of 5 mg per 100 g. The proposed method is applicable to all commercially available potato products and potato starch. © 1990.