LINKAGE OF RECOGNITION AND REPLICATION FUNCTIONS BY ASSEMBLING COMBINATORIAL ANTIBODY FAB LIBRARIES ALONG PHAGE SURFACES

被引：356

作者：

KANG, AS

BARBAS, CF

JANDA, KD

BENKOVIC, SJ

LERNER, RA

机构：

[1] SCRIPPS RES INST, DEPT MOLEC BIOL, LA JOLLA, CA 92037 USA

[2] Scripps Res Inst, DEPT CHEM, LA JOLLA, CA 92037 USA

[3] PENN STATE UNIV, DEPT CHEM, UNIVERSITY PK, PA 16802 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 10期

关键词：

COMBINATORIAL IMMUNOGLOBULIN REPERTOIRE; FILAMENTOUS BACTERIOPHAGE; SURFACE EXPRESSION; COAT PROTEIN-VIII; CATALYTIC ANTIBODIES;

D O I：

10.1073/pnas.88.10.4363

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We describe a method based on a phagemid vector with helper phage rescue for the construction and rapid analysis of combinatorial antibody Fab libraries. This approach should allow the generation and selection of many monoclonal antibodies. Antibody genes are expressed in concert with phage morphogenesis, thereby allowing incorporation of functional Fab molecules along the surface of filamentous phage. The power of the method depends upon the linkage of recognition and replication functions and is not limited to antibody molecules.

引用

页码：4363 / 4366

页数：4

共 23 条

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