PHOSPHORUS STRESS EFFECTS ON ASSIMILATION OF NITRATE

被引:106
作者
RUFTY, TW
MACKOWN, CT
ISRAEL, DW
机构
[1] N CAROLINA STATE UNIV,DEPT CROP SCI,RALEIGH,NC 27695
[2] N CAROLINA STATE UNIV,DEPT BOT,RALEIGH,NC 27695
[3] N CAROLINA STATE UNIV,DEPT SOIL SCI,RALEIGH,NC 27695
[4] UNIV KENTUCKY,DEPT AGRON,LEXINGTON,KY 40546
关键词
D O I
10.1104/pp.94.1.328
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
An experiment was conducted to investigate alterations in uptake and assimilation of NO3- by phosphorus-stressed plants. Young tobacco plants (Nicotians tabacum [L.], cv NC 2326) growing in solution culture were deprived of an external phosphorus (P) supply for 12 days. On selected days, plants were exposed to 15NO3- during the 12 hour light period to determine changes in NO3- assimilation as the P deficiency progressed. Decreased whole-plant growth was evident after 3 days of P deprivation and became more pronounced with time, but root growth was unaffected until after day 6. Uptake of 15NO3- per gram root dry weight and translocation of absorbed 15NO3- out of the root were noticeably restricted in -P plants by day 3, and effects on both increased in severity with time. Whole-plant reduction of 15NO3- and 15N incorporation into insoluble reduced-N in the shoot decreased after day 3. Although the P limitation was associated with a substantial accumulation of amino acids in the shoot, there was no indication of excessive accumulation of soluble reduced-15N in the shoot during the 12 hour 15NO3- exposure periods. The results indicate that alterations in NO3- transport processes in the root system are the primary initial responses limiting synthesis of shoot protein in P-stressed plants. Elevated amino acid levels evidently are associated with enhanced degradation of protein rather than inhibition of concurrent protein synthesis.
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页码:328 / 333
页数:6
相关论文
共 32 条
[1]   RAPID ANALYSIS OF AMINO-ACIDS USING PRE-COLUMN DERIVATIZATION [J].
BIDLINGMEYER, BA ;
COHEN, SA ;
TARVIN, TL .
JOURNAL OF CHROMATOGRAPHY, 1984, 336 (01) :93-104
[2]   EFFECT OF AMINO-COMPOUNDS ON NITRATE UTILIZATION BY ROOTS OF DWARF BEAN [J].
BRETELER, H ;
ARNOZIS, PA .
PHYTOCHEMISTRY, 1985, 24 (04) :653-657
[3]   ANALYSIS BY DIGESTION AND COLORIMETRIC ASSAY OF TOTAL NITROGEN IN PLANT-TISSUES HIGH IN NITRATE [J].
CATALDO, DA ;
SCHRADER, LE ;
YOUNGS, VL .
CROP SCIENCE, 1974, 14 (06) :854-856
[4]  
CLARKSON DT, 1986, FUNDAMENTAL ECOLOGIC, P3
[5]   REGULATION OF ION-TRANSPORT [J].
GLASS, ADM .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1983, 34 :311-326
[6]  
GLASS ADM, 1988, ISI ATL SCI-ANIM PL, V1, P151
[7]   INFLUENCE OF PHOSPHORUS-NUTRITION ON PHOSPHORUS AND NITROGEN-UTILIZATION EFFICIENCIES AND ASSOCIATED PHYSIOLOGICAL-RESPONSES IN SOYBEAN [J].
ISRAEL, DW ;
RUFTY, TW .
CROP SCIENCE, 1988, 28 (06) :954-960
[8]  
Jackson W. A., 1981, Genetic engineering of symbiotic nitrogen fixation and conservation of fixed nitrogen., P517
[9]  
Jackson W.A., 1978, NITROGEN ENV, VVolume 2
[10]   ENZYMIC ASSAY OF 10-7 TO 10-14 MOLES OF SUCROSE IN PLANT-TISSUES [J].
JONES, MGK ;
OUTLAW, WH ;
LOWRY, OH .
PLANT PHYSIOLOGY, 1977, 60 (03) :379-383