5 ADDITIONAL GENES IN THE PULC-O OPERON OF THE GRAM-NEGATIVE BACTERIUM KLEBSIELLA-OXYTOCA UNF5023 WHICH ARE REQUIRED FOR PULLULANASE SECRETION

被引：67

作者：

REYSS, I ^{[1
]}

PUGSLEY, AP ^{[1
]}

机构：

[1] INST PASTEUR,UNITE GENET MOLEC,CNRS,UA 1149,25 RUE DR ROUX,F-75724 PARIS 15,FRANCE

来源：

MOLECULAR & GENERAL GENETICS | 1990年 / 222卷 / 2-3期

关键词：

Klebsiella oxytoca protein secretion; Pullulanase; Secretion gene operon;

D O I：

10.1007/BF00633815

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA sequence analysis, Tn pho and Tn tac-1, mutagenesis, deletion analysis, expression under bacteriophage T7 gene 10 promoter control, subcellular fractionation and complementation tests were used to study the function of DNA located in the centre of the pulC-O operon from Klebsiella oxytoca strain UNF5023. The characterized region of the operon includes five genes (pulG, pulH, pulI, pulJ and pulK) coding for apparently integral inner membrane proteins which are required for pullulanase secretion. The results presented here and previously show that the pulC-O operon contains at least 11 pullulanase secretion genes. © 1990 Springer-Verlag.

引用

页码：176 / 184

页数：9

共 25 条

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