IMMUNOHISTOLOGICAL STAINING OF ANTIGENS ON SEMITHIN SECTIONS OF SPECIMENS EMBEDDED IN PLASTIC (GMA-QUETOL 523)

被引：29

作者：

TAKAMIYA, H ^{[1
]}

BATSFORD, SR ^{[1
]}

TOKUNAGA, J ^{[1
]}

VOGT, A ^{[1
]}

机构：

[1] KYUSHU DENT COLL,DEPT BACTERIOL,KITAKYUSHU,JAPAN

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1979年 / 30卷 / 03期

关键词：

D O I：

10.1016/0022-1759(79)90102-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Glycol methacrylate-Quetol 523, introduced by Kushida (1977) for combined light and electronmicroscopy studies at low magnification, also permits application of immunofluorescence methods to semithin sections. To recover the antigenicity of proteins fixed with formaldehyde, abrupt dehydration before embedding and subsequent treatment of the semithin sections with protease were essential. Post-staining with suitable histological stains allows exact correlation of antigen localization with tissue structure. © 1979.

引用

页码：277 / 288

页数：12

共 9 条

[1] METHOD OF METHACRYLATE-EMBEDDING OF CORPUSCULAR ANTIGEN IN IMMUNOFLUORESCENCE DIAGNOSIS OF FILARIASIS [J].