IDENTIFICATION OF SECRETED BETA-AMYLOID PRECURSOR PROTEIN-BINDING SITES ON INTACT HUMAN FIBROBLASTS

Based upon recent evidence that the secreted form of APP can cause the release of cytokines and elicit other biological activities, we sought to identify whether a receptor could be identified on the surface of cells. The secreted amyloid precursor protein containing the Kunitz domain (scAPP(751)) is identical to protease nexin II, a protease inhibitor which has been shown to form complexes with labeled EGF binding protein that subsequently binds to cells. Results of [I-125]scAPP(751)-trypsin complex incubated with intact fibroblast cells show that the complex appears to bind in a saturable time-dependent and reversible manner. The kinetic constants from the binding studies demonstrate a k(1) = 2.5 x 10(7) M(-1) s(-1) and k(2)=4.7 x 10(-4) s(-1) and thus a KD (=k(2)/k(1)) = 20 PM Furthermore, the complex formation of [I-125]scAPP(751) with a protease appears to be a requirement for optimal binding. The binding affinity of secreted APP demonstrated in this study is consistent with its potency in eliminating a range of biological efforts that have been documented. (C) 1994 Academic Press, Inc.