DETERMINATION OF ASCORBIC-ACID AND DEHYDROASCORBIC ACID IN JUICES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION USING L-CYSTEINE AS PRECOLUMN REDUCTANT

被引：35

作者：

IWASE, H

ONO, I

机构：

[1] Ajinomoto Co., Inc., Central Research Laboratories, Kawasaki-ku, Kawasaki, 210

来源：

JOURNAL OF CHROMATOGRAPHY A | 1993年 / 654卷 / 02期

关键词：

D O I：

10.1016/0021-9673(93)83364-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Determination of ascorbic acid (AA) and dehydroascorbic acid (DHAA) in juices was performed by high-performance liquid chromatography with electrochemical detection using L-cysteine as precolumn reductant. This method was suitable for the determination of AA and total AA (AA + DHAA) in juices. The mild reduction of DHAA to AA with L-cysteine took ca. 15 min, and the retention time of AA was ca. 15 min. The detection limit (signal-to-noise ratio = 2) was ca. 0.15 ng. The method was selective and reproducible (relative standard deviation 2.6-3.2% for AA and 2.1-3.2% for total AA). The calibration graph for AA was linear in the range 0.1-10 ng. The recovery of AA was over 90% by the standard addition method.

引用

页码：215 / 220

页数：6

共 15 条

[1] A HIGHLY SENSITIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY METHOD FOR THE ESTIMATION OF ASCORBIC AND DEHYDROASCORBIC ACID IN TISSUES, BIOLOGICAL-FLUIDS, AND FOODS [J].