TRANSFORMATION OF MOLLICUTES WITH SINGLE-STRANDED TN4001 DNA

被引：6

作者：

KAPKE, PA ^{[1
]}

KNUDTSON, KL ^{[1
]}

MINION, FC ^{[1
]}

机构：

[1] IOWA STATE UNIV SCI & TECHNOL,VET MED RES INST,DEPT MICROBIOL,AMES,IA 50011

来源：

PLASMID | 1994年 / 32卷 / 01期

关键词：

D O I：

10.1006/plas.1994.1048

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Acholeplasma oculi ISM1499 and Mycoplasma gallisepticum were transformed with single-stranded and double-stranded plasmids containing Tn4001. The transposon mobilized to the chromosome using both single-stranded and double-stranded DNA at the same frequency. M. gallisepticum transformed at a 2 log lower frequency than did A. oculi ISM1499. Restriction enzyme digestion of single-stranded DNA indicated homologous base pairing in the inverted repeat regions, which could account for the transpositional activity of single-stranded DNA. (C) 1994 Academic Press, Inc.

引用

页码：85 / 88

页数：4

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