AN ENZYME ASSAY FOR ACETYLCHOLINE

被引：55

作者：

FEIGENSON, ME

SAELENS, JK

机构：

[1] Department of Pharmacology, Geigy Pharmaceuticals, Division of Geigy Chemical Corp., Ardsley

来源：

BIOCHEMICAL PHARMACOLOGY | 1969年 / 18卷 / 06期

关键词：

D O I：

10.1016/0006-2952(69)90262-7

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A specific and sensitive enzymatic assay for acetylcholine has been developed. The acetylcholine was isolated from tissue extracts by electrophoresis, hydrolyzed to choline, and reacetylated by the transfer of the acetyl-14C group of acetyl-14C-coenzyme A to the choline molecule by the enzyme, choline acetyltransferase. The rate of formation of the product, acetyl-14C-cholinc, was linearly related to the concentration of substrate choline up to 30.0 μg per ml. The assay can be used to measure acetylcholine in as little as 10 mg brain tissue (0.01 μg acetylcholine). © 1969.

引用

页码：1479 / +

页数：1

共 9 条

[1] INHIBITION OF ACETYLCHOLINE SYNTHESIS IN NERVOUS TISSUE BY SOME QUARTERNARY COMPOUNDS [J].