ELECTROPHORETIC FORMS OF LYSOZYME ACTIVITY IN VARIOUS PLANT-SPECIES

被引:20
作者
AUDY, P
LEQUERE, D
LECLERC, D
ASSELIN, A
机构
[1] Département de phytologie, Faculté des sciences de l'agriculture et de l'alimentation, Université Laval
基金
加拿大自然科学与工程研究理事会;
关键词
bryophytes; chitinase; isoforms; Lysozyme; polyacrylamide gel electrophoresis; pteridophytes; spermatophytes;
D O I
10.1016/0031-9422(90)85419-G
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Proteins from leaf, seed, fruit and vegetable clarified homogenates were first separated in native polyacrylamide gels specific for basic proteins. They were then transferred to an overlay gel containing Micrococcus cells as substrate for detection of lysozyme activity. Extracts from 410 plant species representing 116 families were tested and lysozyme activity was found in 168 species from 71 families. One to five electrophoretic forms of lysozyme activity were observed. The relative electrophoretic mobility and intensity of each isoform was compared with known amounts of hen egg white lysozyme as reference lysozyme. This is the first report of the presence of lysozyme activity in gymnosperms and lower plants. In 20μl extracts, isoforms with activity corresponding to more than 10 μg of purified hen egg white lysozyme were found in Lychnis chaledonica, Phaseolus coccineus, Petroselinum crispum, Phytolacca americana, Adonis vernalis, Dictamus albus and Hyoscyamus niger leaf extracts, and Rumex acetosa and Punica granatum seed extracts. © 1990.
引用
收藏
页码:1143 / 1159
页数:17
相关论文
共 44 条
  • [1] IMMUNOCYTOCHEMICAL LOCALIZATION OF A WHEAT-GERM LYSOZYME IN WHEAT EMBRYO AND COLEOPTILE CELLS AND CYTOCHEMICAL STUDY OF ITS INTERACTION WITH THE CELL-WALL
    AUDY, P
    BENHAMOU, N
    TRUDEL, J
    ASSELIN, A
    [J]. PLANT PHYSIOLOGY, 1988, 88 (04) : 1317 - 1322
  • [2] PURIFICATION AND CHARACTERIZATION OF A LYSOZYME FROM WHEAT-GERM
    AUDY, P
    TRUDEL, J
    ASSELIN, A
    [J]. PLANT SCIENCE, 1988, 58 (01) : 43 - 50
  • [3] A ONE-STEP PURIFICATION OF A PLANT LYSOZYME FROM INVITRO CULTURES OF RUBUS-HISPIDUS
    BERNASCONI, P
    PILET, PE
    JOLLES, P
    [J]. FEBS LETTERS, 1985, 186 (02) : 263 - 266
  • [4] PURIFICATION AND N-TERMINAL AMINO-ACID-SEQUENCE OF A BASIC LYSOZYME FROM PARTHENOCISSUS-QUINQUIFOLIA CULTURED INVITRO
    BERNASCONI, P
    LOCHER, R
    PILET, PE
    JOLLES, J
    JOLLES, P
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1987, 915 (02) : 254 - 260
  • [5] TURNIP LYSOZYME .77.
    BERNIER, I
    VANLEEMP.E
    HORISBER.M
    BUSH, DA
    JOLLES, P
    [J]. FEBS LETTERS, 1971, 14 (02) : 100 - &
  • [6] CHITINASE IN BEAN-LEAVES - INDUCTION BY ETHYLENE, PURIFICATION, PROPERTIES, AND POSSIBLE FUNCTION
    BOLLER, T
    GEHRI, A
    MAUCH, F
    VOGELI, U
    [J]. PLANTA, 1983, 157 (01) : 22 - 31
  • [7] Boller T, 1985, CELLULAR MOL BIOL PL, P247
  • [8] COMPARISON OF SOME MOLECULAR, ENZYMATIC AND ANTIFUNGAL PROPERTIES OF CHITINASES FROM THORN-APPLE, TOBACCO AND WHEAT
    BROEKAERT, WF
    VANPARIJS, J
    ALLEN, AK
    PEUMANS, WJ
    [J]. PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 1988, 33 (03) : 319 - 331
  • [9] DETERMINATION OF LYSOZYME IN RAW FRUITS AND VEGETABLES
    CHANDAN, RC
    EREIFEJ, KI
    [J]. JOURNAL OF FOOD SCIENCE, 1981, 46 (04) : 1278 - 1279
  • [10] ACTION OF VARIOUS LYSOZYMES ON CHITOPENTAOSE
    CHARLEMAGNE, D
    JOLLES, P
    [J]. FEBS LETTERS, 1972, 23 (02) : 275 - +