FAST SOLID SUPPORT DETECTION OF PCR AMPLIFIED VIRAL-DNA SEQUENCES USING RADIOIODINATED OR HAPTEN LABELED PRIMERS

被引：35

作者：

SAUVAIGO, S

FOUQUE, B

ROGET, A

LIVACHE, T

BAZIN, H

CHYPRE, C

TEOULE, R

机构：

[1] CEN,CHIM LABS,DEPT RECH FONDAMENTALE,85X,F-38041 GRENOBLE,FRANCE

[2] CIS BIO INT,SUNDES MOLEC LAB,GRENOBLE,FRANCE

来源：

NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 11期

关键词：

D O I：

10.1093/nar/18.11.3175

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ollgonucleotides with novel modifications have been synthesized and Incorporated Into enzymatlcally amplified DNA sequences. They allow the fast detection of viral DNA sequences after two rounds ofamplification. The hybrids formed are Immobilized by affinity on coated tubes and detected by direct beta (32P) or gamma (125I) counting or by colorimetrlc revelation. The effect of a dilution step between the two amplifications Is studied to obtain optimal sensitivity and specificity. This test Is used to detect Human Papillomavirus types 16 and 18 in cells and biopsiesand for the specific colorimetric detection of HIV1 in extracted DNA. © 1990 Oxford University Press.

引用

页码：3175 / 3183

页数：9

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