PHOSPHOLIPASE-C ISOZYMES IN NEURONS AND GLIAL-CELLS IN CULTURE - AN IMMUNOCYTOCHEMICAL AND IMMUNOCHEMICAL STUDY

被引：32

作者：

MIZUGUCHI, M ^{[1
]}

YAMADA, M ^{[1
]}

KIM, SU ^{[1
]}

RHEE, SG ^{[1
]}

机构：

[1] NHLBI,BIOCHEM LAB,BETHESDA,MD 20892

来源：

BRAIN RESEARCH | 1991年 / 548卷 / 1-2期

关键词：

PHOSPHOINOSITIDE 2ND-MESSENGER SYSTEM; TISSUE CULTURE; IMMUNOCYTOCHEMISTRY; IMMUNOBLOTTING; NEURON; GLIA;

D O I：

10.1016/0006-8993(91)91103-8

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Expression of 3 brain isozymes of phospholipase (PLC-beta, PLC-gamma and PLC-sigma) was investigated in relation to cell types found in rat CNS cultures. Immunoreactivity of cultured neurons, astrocytes and oligodendrocytes was demonstrated for all of the 3 isozymes by immunocytochemical staining and immunoblotting, with some differences in reaction intensity. Immunoblotting revealed that the level of expression was neurons > oligodendrocytes > astrocytes for PLC-beta and PLC-gamma, and astrocytes > oligodendrocytes > neurons for PLC-sigma.

引用

页码：35 / 40

页数：6

共 35 条

[1] DIFFERENTIATED RAT GLIAL CELL STRAIN IN TISSUE CULTURE [J].