CRYSTALLIZATION AND PRELIMINARY CRYSTALLOGRAPHIC STUDIES OF GLUCOSAMINE-6-PHOSPHATE DEAMINASE FROM ESCHERICHIA-COLI K12

被引：9

作者：

HORJALES, E

ALTAMIRANO, MM

CALCAGNO, ML

DAUTER, Z

WILSON, K

GARRATT, RC

OLIVA, G

机构：

[1] UNIV SAO PAULO,INST FIS & QUIM SAO CARLOS,CAIXA POSTAL 369,BR-13560 SAO CARLOS,SP,BRAZIL

[2] UNIV REPUBLICA,FAC CIENCIAS,INST FIS,MONTEVIDEO,URUGUAY

[3] NATL AUTONOMOUS UNIV MEXICO,FAC MED,DEPT BIOQUIM,MEXICO CITY 04510,DF,MEXICO

[4] DESY,EUROPEAN MOLEC BIOL LAB OUTSTN,W-2000 HAMBURG 52,GERMANY

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1992年 / 226卷 / 04期

基金：

巴西圣保罗研究基金会;

关键词：

DEAMINASE; GLUCOSAMINE; HEXAMER; ALLOSTERY; CRYSTALLIZATION;

D O I：

10.1016/0022-2836(92)91068-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hexameric glucosamine-6-phosphate deaminase from Escherichia coli has been crystallized isomorphously with both phosphate and ammonium sulphate as precipitants, over a wide pH range (6·0 to 9·0). The crystals belong to space ropu R32 and the cell parameters in the hexagonal settings are a = b = 125·9 A ̊ and c = 223·2 A ̊. A complete native data set was collected to 2·1 Å resolution. Self-rotation function studies suggest that the hexamers sit on the 3-fold axis and have point group symmetry 32, with a non-crystallographic dyad relating two monomers linked by an interchain disulfide bridge. A possible packing for the unit cell is proposed. © 1992.

引用

页码：1283 / 1286

页数：4

共 20 条

[1] SULFHYDRYL-GROUPS OF GLUCOSAMINE-6-PHOSPHATE ISOMERASE DEAMINASE FROM ESCHERICHIA-COLI [J].