FLOW-CYTOMETRY ANALYSIS OF THE EXPRESSION OF NEUTROPHIL FMLP RECEPTORS

被引：27

作者：

ALLEN, CA ^{[1
]}

BROOM, MF ^{[1
]}

CHADWICK, VS ^{[1
]}

机构：

[1] UNIV OTAGO,SCH MED,DEPT EXPTL MED,POB 913,DUNEDIN,NEW ZEALAND

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1992年 / 149卷 / 02期

关键词：

FLOW CYTOMETRY; NEUTROPHIL; FORMYL-METHIONINE-LEUCINE-PHENYLALANINE RECEPTOR;

D O I：

10.1016/0022-1759(92)90246-P

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The number of cell surface FMLP receptors expressed by peripheral blood neutrophils taken from healthy individuals was quantitated using a direct fluorescence label analysed on the FACScan. Receptor numbers, assayed on cells from heparinised blood prepared at 4-degrees-C or 37-degrees-C or in the presence of LPS (30 ng/ml) were 12,540 +/- SD 2413, 18,588 +/- 4938 and 45,041 +/- 10,744 respectively. Anticoagulation with EGTA resulted in 25-30% greater basal receptor numbers but had no effect on receptor level after maximal stimulation with LPS. Double labelling with a neutrophil-specific mAb showed that the entire neutrophil population expressed FMLP receptors.

引用

页码：159 / 164

页数：6

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