ANALYSIS OF SALMONELLA-TYPHIMURIUM HISD3052 REVERTANTS - THE USE OF OLIGODEOXYRIBONUCLEOTIDE COLONY HYBRIDIZATION, PCR, AND DIRECT SEQUENCING IN MUTATIONAL ANALYSIS

被引：40

作者：

KUPCHELLA, E ^{[1
]}

CEBULA, TA ^{[1
]}

机构：

[1] US FDA,CTR FOOD SAFETY & APPL NUTR,DIV MICROBIOL,MOLEC BIOL BRANCH,HFF235,WASHINGTON,DC 20204

来源：

ENVIRONMENTAL AND MOLECULAR MUTAGENESIS | 1991年 / 18卷 / 04期

关键词：

SEQUENCE ANALYSIS; DNA PROBES; FRAMESHIFT MUTATION; SALMONELLA AMES;

D O I：

10.1002/em.2850180404

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

A rapid method for determining the DNA sequences of Salmonella typhimurium hisD3052 revertants is presented. DNA colony hybridization was used to analyze revertants previously studied by Isono and Yourno [Proc Natl Acad Sci USA 71:1612-1617,1974]. Synthetic oligodeoxyribonucleotide probes (18-mers) were able to distinguish sequences that differed by a single base pair. Mutant his sequences not identified by probing analysis were amplified using polymerase chain reaction (PCR) and directly sequenced. The combined use of DNA-colony hybridization and direct sequencing offers a precise and rapid means for the molecular characterization of hisD3052 revertants.

引用

页码：224 / 230

页数：7

共 30 条

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