SIMULTANEOUS DETECTION OF TOXIN-A AND TOXIN-B GENETIC-DETERMINANTS OF CLOSTRIDIUM-DIFFICILE USING THE MULTIPLEX POLYMERASE CHAIN-REACTION

被引：18

作者：

MCMILLIN, DE ^{[1
]}

MULDROW, LL ^{[1
]}

LAGGETTE, SJ ^{[1
]}

机构：

[1] CLARK ATLANTA UNIV,ATLANTA,GA 30314

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 1992年 / 38卷 / 01期

关键词：

CLOSTRIDIUM-DIFFICILE; POLYMERASE CHAIN REACTION; BACTERIAL TOXINS;

D O I：

10.1139/m92-013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A multiplex polymerase chain reaction was developed to simultaneously detect the presence of toxin A and toxin B genes of Clostridium difficile. A 1050-bp fragment of the toxin B gene and a 1217-bp fragment of the toxin A gene were amplified from 42 toxic strains of C. difficile; however, from 10 nontoxic strains the toxin gene fragments were not amplified; these data demonstrate that this multiplex polymerase chain reaction procedure can be used to differentiate between toxic and nontoxic strains. This sensitive and specific multiplex polymerase chain reaction for C. difficile toxins may prove to be a valuable diagnostic procedure.

引用

页码：81 / 83

页数：3

共 10 条

[1] NUCLEOTIDE-SEQUENCE OF CLOSTRIDIUM-DIFFICILE TOXIN-B GENE [J].