THE RHIZOBIUM-LEGUMINOSARUM BIOVAR PHASEOLI GLNT GENE, ENCODING GLUTAMINE SYNTHETASE-III

被引：28

作者：

CHIURAZZI, M

MEZA, R

LARA, M

LAHM, A

DEFEZ, R

IACCARINO, M

ESPIN, G

机构：

[1] UNIV NACL AUTONOMA MEXICO,INST BIOTECNOL,APARTADO POSTAL 510-3,CUERNAVACA 62271,MEXICO

[2] CNR,IST INT GENET & BIOFIS,I-80125 NAPLES,ITALY

[3] IRBM,I-00040 POMEZIA,ITALY

来源：

GENE | 1992年 / 119卷 / 01期

关键词：

RECOMBINANT DNA; NUCLEOTIDE SEQUENCE; KLEBSIELLA-PNEUMONIAE; PLASMID VECTOR;

D O I：

10.1016/0378-1119(92)90060-3

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Plasmid pGE203 contains the Rhizobium leguminosarum biovar phaseoli glnT locus. Glutamine synthetase III (GSIII) was purified from a glutamine auxotrophic strain of Klebsiella pneumoniae carrying this plasmid. Sequencing of a 2.4-kb fragment containing the glnT locus reveals an open reading frame of 435 amino acids (aa), whose first eight aa are identical to those determined from pure GSIII by direct aa sequencing, thus confirming that glnT indeed codes for GSIII activity. The comparison of the GSIII aa sequence with the reported sequence of GSs from other organisms shows a significant degree of homology. Since the three-dimensional structure of GS from Salmonella typhimurium is known, a three-dimensional model of GSIII was built by homology.

引用

页码：1 / 8

页数：8

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[1] NOVEL SUBUNIT SUBUNIT INTERACTIONS IN THE STRUCTURE OF GLUTAMINE-SYNTHETASE [J].