CLUSTERING OF MONOCLONAL-ANTIBODIES RECOGNIZING DIFFERENT MEMBERS OF THE WC1 GENE FAMILY

被引:29
作者
MACHUGH, ND
WIJNGAARD, PLJ
CLEVERS, HC
DAVIS, WC
机构
[1] UNIV UTRECHT HOSP,DEPT IMMUNOL,3511 GV UTRECHT,NETHERLANDS
[2] WASHINGTON STATE UNIV,DEPT VET MICROBIOL PATHOL,PULLMAN,WA 99164
关键词
D O I
10.1016/0165-2427(93)90176-5
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Mouse L cell lines expressing two different bovine WC1 glycoproteins were produced by transfection of the cells with the corresponding cDNAs. The cell lines were used to analyze the reactivities of 67 monoclonal antibodies (mAbs) which recognize bovine gamma/delta T cells. The results indicated that preliminary clustering of mAbs can be achieved based on their recognition of epitopes expressed on all gene products, or of epitopes encoded by individual members of the gene family. The studies also showed that at least three members of the WC1 gene family are expressed, although it is not yet known how many can be expressed by individual bovine gamma/delta T cells. Final clustering of the WC1 mAbs will not be possible until the exact number of expressed gene products is known, and the reactivities of the mAbs with these products have been analyzed.
引用
收藏
页码:155 / 160
页数:6
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