IMPROVED EXTRACTION AND HPLC METHODS FOR ERGOVALINE FROM PLANT-MATERIAL AND RUMEN FLUID

被引：35

作者：

CRAIG, AM

BILICH, D

HOVERMALE, JT

WELTY, RE

机构：

[1] College College of Veterinary Medicine, Oregon State University, Corvallis

[2] USDA-ARS National Forage Seed Production Research Center, Department of Botany and Plant Pathology, Oregon State University, Corvallis

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 1994年 / 6卷 / 03期

关键词：

D O I：

10.1177/104063879400600311

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Ergovaline, the main ergopeptine alkaloid produced in tall fescue infected with Acremonium coenophialum, is known to cause tall fescue toxicosis. Current methods in use for quantifying ergovaline in plant material have several disadvantages, including large solvent volumes and long analysis time. We report here improvements in extraction and cleanup and the high-pressure liquid chromatographic methods. Our improvements include a 24-hour extraction time, the use of smaller solvent volumes during sample preparation, and fast analysis on the polymeric reverse-phase column. In addition to allowing the analysis of large batches to assist practitioners in the accurate diagnosis of fescue toxicosis, our method is also easily modified for other matrices, such as rumen fluid.

引用

页码：348 / 352

页数：5

共 12 条

[1]

Hemken R.W., Bull J.A., Boling J.A., Et al., Summer fescue toxicosis in lactating dairy cows and sheep fed experimental strains of ryegrass-tall fescue hybrids, J Anim Sci, 49, pp. 641-646, (1979)

[2]

Hill N.S., Physiology of plant-endophyte interactions: implications and use of endophytes in plant breeding, Proc Int Symp Acremonium/Grass Interact, 2, pp. 161-169, (1993)

[3]

Hill N.S., Rottinghaus G.E., Agee C.S., Schultz L.M., Simplified sample preparation for HPLC analysis of ergovaline in tall fescue, Crop Sci, 33, pp. 331-333, (1993)

[4]

Hill N.S., Stringer W.C., Rottinghaus G.E., Et al., Growth, morphological and chemical component responses of tall fescue to Acremonium coenophialum, Crop Sci, 30, pp. 156-161, (1990)

[5]

Lyons P.C., Plattner R.D., Bacon C.W., Occurrence of peptide and clavine ergot alkaloids in tall fescue grass, Science, 232, pp. 487-489, (1986)

[6]

Rottinghaus G.E., Gamer G.B., Cornell C.N., Ellis J.L., HPLC method for quantitating ergovaline in endophyte-infected tall fescue: seasonal variation of ergovaline levels in stems with leaf sheaths, leaf blades, and seed heads, J Agric Food Chem, 39, pp. 112-115, (1991)

[7]

Rottinghaus G.E., Schultz L.M., Ross P.F., Hill N.S., An HPLC method for the detection of ergot in ground and pelleted feeds, J Vet Diagn Invest, 5, pp. 242-247, (1993)

[8]

Schmidt S.P., Hoveland C.S., Clark E.M., Et al., Association of an endophytic fungus with fescue toxicity in steers fed Kentucky 31 tall fescue seed or hay, J Anim Sci, 55, pp. 1259-1263, (1982)

[9]

Solomons R.N., Oliver J.W., Linnabary R.D., Reactivity of dorsal pedal vein of cattle to selected alkaloids associated with Acremonium coenophialum-infected fescue grass, Am J Vet Res, 50, pp. 235-238, (1989)

[10]

Wachenheim D.E., Blythe L.L., Craig A.M., Effects of antibacterial agents on in vitro ovine ruminal biotransformation of the hepatotoxic pyrrolizidine alkaloid jacobine, Appl Environ Microbiol, 58, pp. 2559-2564, (1992)

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