FLUOROMETRIC ASSAY OF CORTICOSTERONE IN PRESENCE OF CHOLESTEROL BACKGROUND

被引：6

作者：

FLACK, JD

STOCKHAM, MA

机构：

[1] Department of Pharmacology, School of Pharmacy, London University, London, England, Brunswick Square

[2] Sherrington School of Physiology, St. Thomas's Hospital Medical School, London, England

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 27卷 / 01期

关键词：

D O I：

10.1016/0003-2697(69)90217-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An analysis of the fluorescent spectra of corticosterone and cholesterol sulfuric acid products using orthogonal polynomials has enabled a calculation of corticosterone contributions in the presence of large amounts of cholesterol. The range of percentage recovery of corticosterone from mixtures with cholesterol with this method was 80 to 120%, whereas the same data gave a range of 95 to 310% when only the values at 525 mμ, the fluorescent peak for corticosterone, were utilized. © 1968.

引用

页码：47 / &

共 16 条

[1] EXPERIMENTAL BASIS FOR SELECTING THE OPTIMAL CONDITIONS FOR QUANTITATIVE FLUOROMETRY OF NATURAL ESTROGENS [J].