SELECTIVE CLEAVAGE OF 28S RIBOSOMAL-RNA VARIABLE REGIONS V3 AND V13 IN MYELOID-LEUKEMIA CELL APOPTOSIS

被引：53

作者：

HOUGE, G ^{[1
]}

DOSKELAND, SO ^{[1
]}

BOE, R ^{[1
]}

LANOTTE, M ^{[1
]}

机构：

[1] UNIV BERGEN, DEPT CELL BIOL & ANAT, N-5009 BERGEN, NORWAY

来源：

FEBS LETTERS | 1993年 / 315卷 / 01期

关键词：

MYELOID LEUKEMIA; APOPTOSIS; 28S RIBOSOMAL RNA;

D O I：

10.1016/0014-5793(93)81123-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Vigorous apoptosis is induced 3-4 hours after activation of cAMP-dependent protein kinase I in the rat myeloid leukemia cell line IPC-81 [J. Cell. Physiol. 146 (1991) 73-80]. We will report a novel feature of apoptosis in these cells: a selective and temporarily ordered cleavage within the two largest 28S ribosomal RNA variable regions (V3 and V13). The cleavage of 28S rRNA coincided with internucleosomal DNA fragmentation and cessation of cellular protein synthesis. The implication of 28S variable regions as targets in apoptosis is a clue to the function of these so far apparently superfluous parts of eukaryotic ribosomes.

引用

页码：16 / 20

页数：5

共 29 条

[1] THE PROTEIN PHOSPHATASE INHIBITOR OKADAIC ACID INDUCES MORPHOLOGICAL-CHANGES TYPICAL OF APOPTOSIS IN MAMMALIAN-CELLS [J].

BOE, R ;

GJERTSEN, BT ;

VINTERMYR, OK ;

HOUGE, G ;

LANOTTE, M ;

DOSKELAND, SO .

EXPERIMENTAL CELL RESEARCH, 1991, 195 (01) :237-246

[2] SCRAMBLED RIBOSOMAL-RNA GENE PIECES IN CHLAMYDOMONAS REINHARDTII MITOCHONDRIAL-DNA [J].

BOER, PH ;

GRAY, MW .

CELL, 1988, 55 (03) :399-411

[3] XENOPUS-LAEVIS-28S RIBOSOMAL-RNA - A SECONDARY STRUCTURE MODEL AND ITS EVOLUTIONARY AND FUNCTIONAL IMPLICATIONS [J].

CLARK, CG ;

TAGUE, BW ;

WARE, VC ;

GERBI, SA .

NUCLEIC ACIDS RESEARCH, 1984, 12 (15) :6197-6220

[4] DEVELOPMENTAL CELL-DEATH - MORPHOLOGICAL DIVERSITY AND MULTIPLE MECHANISMS [J].

CLARKE, PGH .

ANATOMY AND EMBRYOLOGY, 1990, 181 (03) :195-213

[5]

COHEN JJ, 1991, ADV IMMUNOL, V50, P55

[6] MOLECULAR EVOLUTION - RDNA WORLD FALLING TO PIECES [J].

DOVER, GA .

NATURE, 1988, 336 (6200) :623-624

[7] STUDIES ON ACCESSIBILITY OF RIBOSOMES TO INACTIVATION BY TOXIC LECTINS ABRIN AND RICIN [J].

FODSTAD, O ;

OLSNES, S .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1977, 74 (02) :209-215

[8] EVOLUTION OF EUKARYOTIC RIBOSOMAL-RNA - CONSTRAINTS IMPOSED BY RNA INTERACTIONS [J].

GERBI, SA ;

JEPPESEN, C ;

STEBBINSBOAZ, B ;

ARES, M .

COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1987, 52 :709-719

[9] HOMING OF A DNA ENDONUCLEASE GENE BY MEIOTIC GENE CONVERSION IN SACCHAROMYCES-CEREVISIAE [J].

GIMBLE, FS ;

THORNER, J .

NATURE, 1992, 357 (6376) :301-306

[10] HUMAN 28S RIBOSOMAL-RNA SEQUENCE HETEROGENEITY [J].

GONZALEZ, IL ;

SYLVESTER, JE ;

SCHMICKEL, RD .

NUCLEIC ACIDS RESEARCH, 1988, 16 (21) :10213-10224

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