OVERPRODUCTION OF EXTRACELLULAR ENDOGLUCANASE BY GENETICALLY ENGINEERED BACILLUS-SUBTILIS

被引：16

作者：

KIM, JH ^{[1
]}

PACK, MY ^{[1
]}

机构：

[1] KOREA ADV INST SCI & TECHNOL,DEPT BIOTECHNOL,YUSUNG GU,TAEJON 305701,SOUTH KOREA

来源：

BIOTECHNOLOGY LETTERS | 1993年 / 15卷 / 02期

关键词：

D O I：

10.1007/BF00133012

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Overproduction of extracellular endoglucanase was attempted by modifying promoter region of an endoglucanase gene cloned from Bacillus subtilis BSE616 and expressing in B. sublilis DB104. A strong promoter was cloned from B. subtilis 168 chromosomal DNA and fused to the endoglucanase gene after removing its native promoter. An effective Shine-Dalgarno sequence was inserted between the promoter and the endoglucanase structural gene. The modified gene was expressed well in B. subtilis and produced 265 units of endoglucanase per mg protein that is 60 % of total protein which was secreted into culture medium.

引用

页码：133 / 138

页数：6

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